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Fig. 3 | BMC Bioinformatics

Fig. 3

From: Genome-wide functional analysis using the barcode sequence alignment and statistical analysis (Barcas) tool

Fig. 3

Comparison between perfect and imperfect matching. With only forward barcode sequences, reads were mapped to 1,230 shRNA sequences from the TRC library. Total read counts are 22,497,317 (NE), 17,003,721 (ERG) and 22,573,358 (MRG). In the imperfect matching, at most, two base pair errors, mismatches and indels, were allowed. Compared to perfect matching, imperfect matching mapped an additional 3.64%, 3.55% and 2.59% reads in NE, ERG and MRG samples. While most barcodes showed similar mapped counts between perfect matching and imperfect matching, several barcodes were mapped much better in imperfect matching

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BMC Bioinformatics

ISSN: 1471-2105

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