Fig. 3

Data correction for acetyl-CoA and HMG-CoA using FluxFix. Input data as signal intensity (left y-axis) are in black and grey and output percent molar enrichment data (right y-axis) are in red. Molar enrichment from [¹³C]-glucose occurs in the M2 for acetyl-CoA and M2, M4 and M6 isotopologues for HMG-CoA. This incorporation of glucose is consistent with the known metabolic pathways by which glucose carbon is incorporated in pairs and to a maximum of two atoms for acetyl-CoA and six atoms for HMG-CoA. Data is from three replicate samples, error bars are standard deviation