Fig. 2
From: PARRoT- a homology-based strategy to quantify and compare RNA-sequencing from non-model organisms
PARRoT workflow. PARRoT includes the following analysis steps: 1) de novo assembly of pooled RNA-Seq data; 2) functional annotation and homolog search; 3) generated virtual transcripts; 4) mapping the sequence reads; 5) quantification of each transcript contig by calculating the RC (number of mapped read counts), eRPKM (estimated RPKM) and eTPM (estimated TPM) in each transcriptome dataset; and 6) show the expression level of contig or virtual transcript for two datasets together with their functional annotations