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Table 4 Effect of concatenating assemblies on CDS length

From: Combining independent de novo assemblies optimizes the coding transcriptome for nonconventional model eukaryotic organisms

  

Illumina derived datasets

Simulated datasets

 

Assembler

L. stagnalis

S. cerevisiae

Caenorhabditis sp.

D. simulans

H. rhamnoides

N. benthamiaana

D. melanogaster

C.elegans

Number of extended CDSsa

CLC

8,195 (38%)

1,333 (23%)

7,255 (44%)

5,490 (34%)

11,508 (38%)

24,218 (47%)

1,888 (23%)

1,534 (13%)

IDBA_tran

13,401 (27%)

957 (16%)

11,717 (38%)

7,586 (25%)

11,427 (24%)

48,830 (36%)

1,960 (23%)

2,054 (18%)

Trinity

23,532 (34%)

2,879 (27%)

28,918 (44%)

11,638 (33%)

21,032 (35%)

78,038 (40%)

7,817 (38%)

5,808 (33%)

Cumulated extended CDS length (bp)

CLC

9,289,434

913,872

6,758,496

5,921,460

6,816,978

23,238,501

2,662,092

1,107,756

IDBA_tran

23,112,789

121,116

9,431,703

5,795,478

5,143,413

36,408,087

2,311,569

1,433,037

Trinity

33,055,113

2,749,041

30,317,865

14,029,893

16,390,158

53,633,142

10,201,314

4,291,554

Mean extended CDS length (bp)

CLC

1,134

686

932

1,079

592

960

1,410

722

IDBA_tran

1,725

127

805

764

450

746

1,179

698

Trinity

1,405

955

1,048

1,206

779

687

1,305

739

  1. aThe proportion of CDSs with an extension are indicated in brackets