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Table 2 Number of variants detected among 24 soybean, barley, and potato samples. The sequencing platform, number of reads, filtering options, and genotype accuracy for each dataset are also provided

From: Fast-GBS: a new pipeline for the efficient and highly accurate calling of SNPs from genotyping-by-sequencing data

 

Filtering optionsa

Name

Sequencing platform

Restriction enzyme

Number of reads

minNR

MinMAF

MaxMD (%)

Number of variants

Accuracy (%)

Soybean

Illumina

ApeKI

42 M

2

0.04

80

35 k

98.7

Barley

Ion Torrent

MspI/PstI

72 M

2

0.04

80

32 k

95.2

Potato

Illumina

MspI/PstI

43 M

11

0.04

20

38 k

94.0

  1. aFiltering options: minNR minimum number of reads to call a variant (depth), MinMAF minimum minor allele frequency, and MaxMD maximum missing data allowed