Fig. 10

Relative emission SPDs measured from the images generated from rendering the four fluorescent tissue blocks tagged with Alexa Fluor 350, – 488, – 568 and – 633 at different excitation wavelengths between 300 and 700 nm. The profiles are normalized to the SPD measured at maximum excitation wavelength for each respective dye. The SPDs are detected at 1024 spectral samples per pixel. Notice the relation between the amplitude of the excitation spectrum of each dye at the exciting wavelength and maximum amplitude of measured SPD