Table 1 List of specific (G1) and single (S1) pairs of primers for in vitro validation
From: TipMT: Identification of PCR-based taxon-specific markers
Group | Species | Primer Name | Sequence Forward (5’ - > 3’) | Sequence Reverse (5’ - > 3’) | Amplicon (pb) |
|---|---|---|---|---|---|
G1 | L. amazonensis | Lam.A480-1 | GCGCGCGAGAAAATAGAGAC | GGGCGTCGTCGATATCTGTT | 379 |
L. braziliensis | LbrM.11.1130-0 | ACAGAACAATCAGGCCCGAG | GCTATCGGACGCCTCATCAA | 244 | |
L. infantum | LinJ.36.1330-0 | AGTTCCTTTGTTGTTGTGTTTCGT | TTATCTCTCCCGTCCCTCCG | 334 | |
S1 | L. infantum, L. braziliensis and L. major | LmjF29-1 | GCGGTGCTTGAATCACGTTT | GCGGTGTTTACATGACGACG | 307-322-337 |