Fig. 1
From: Power analysis for RNA-Seq differential expression studies
Mean-dispersion functional form for simulations. DESeq2 method was applied on a pilot data of unpublished canine thyroid RNA-Seq data set for setting up simulation parameters. The plot shows the estimated mean-dispersion function form (red dots) relative to the mean of the normalized counts. Black dots represent per-gene estimates of the dispersion while blue dots represent moderated estimates calculated by DESeq2. The fitted functional form and a lower and higher dependency functional forms were used in the simulation studies