Fig. 5
From: quantGenius: implementation of a decision support system for qPCR-based gene quantification
Importance of implemented QC-DSS as shown in the gene expression use case. Expression of two target genes (PR1-b, upper panel and Glu-II, lower panel) was analysed in mock- and virus-inoculated potato plants at one, three and six days post infection (dpi). EF-1 and COX were used for normalization [16], (Additional file 6). Relative expression values obtained by quantGenius (cross) are compared to the ones obtained using standard curve quantification without QC performed (std curve, circle) and the ΔΔCq quantification approach (ΔΔCq, diamond). To get comparable values in the three approaches, the results of each approach were normalized to one of the samples (virus 3dpi 2) and then scaled to the average expression of the first experimental group (mock 1dpi). a arrows - examples of samples with Cq values near LOQ showing high variability among the quantification approaches used. b arrows - examples of outlier samples with an efficiency problem detected in either the target or the reference gene where results are not calculated in quantGenius