Fig. 13

Comparison of CRISPRn and CRISPRi screen performance for genes with different knockdown-phenotype relationships. Simulations of FACS-based screens were run for 100× representation at the transfection, bottleneck and sequencing stages. The simulated genome contained 75% of genes with a linear knockdown-phenotype relationship and 25% of genes with a sigmoidal knockdown-phenotype relationship, as defined in the Implementation section. Performance in hit detection was quantified as AUPRC either for all genes, or only for linear or sigmoidal genes. Lines and light margins represent means and 99% confidence intervals, respectively, for 100 independent simulation runs