From: Network design and analysis for multi-enzyme biocatalysis
Position | Criterion | Comment |
---|---|---|
1 | Number of active reactions | Shorter pathways are |
favourable | ||
2 | Candidate starts with basic metabolites only | ’yes’ is preferred |
3 | Number of reactions without Δ r G | As few as possible |
4 | \(\sum (\Delta _{r}G + |\Delta _{r}G|)\) | Preferably all Δ r G are |
negative | ||
5 | \(\sum \Delta _{r}G\) | Negative is preferred |
6 | Number of heterologous enzymes | As few as possible |
7 | Number of cofactors | As few as possible |