Fig. 2
From: High-throughput PCR assay design for targeted resequencing using primerXL
Amplicon and sequencing statistics. Graphical representation of the amplicon and sequencing statistics of four sequencing projects. a Target coverage efficiency, b Distribution of the amplicon sequence, c Schematic displaying the different on- and off-target portions of an amplicon, d Cumulative distribution of the amplicon length, e Cumulative distribution of the Cq values (amplicons of Project 2 were tested by regular PCR), f Cumulative distribution of the end-point fluorescence and g Cumulative distribution of the sequencing coverage