Table 1 Sample experimental data from a single qPCR plate for analysis. Hypothetical data are used to show the results of a plate experiment examining the expression of a gene of interest (g) and two reference genes (ref1 and ref2) for two sample types, A and B. The controls for the plate experiment are not shown. \( Mean{C}_q^{(w)} \) represents the the arithmetic \( Mean{C}_q^{(w)} \) across the three technical replicates
Well efficiency (E) | Gene | C q | log(E) | log(E) ∙ C q | \( Mean{C}_q^{(w)} \) | |
|---|---|---|---|---|---|---|
Sample type A | 1.844 | g | 31.246 | 0.266 | 8.303 | 8.397 |
Sample type A | 1.843 | g | 31.490 | 0.265 | 8.360 | |
Sample type A | 1.836 | g | 32.316 | 0.264 | 8.527 | |
Sample type B | 1.839 | g | 32.565 | 0.265 | 8.647 | 8.611 |
Sample type B | 1.834 | g | 32.782 | 0.263 | 8.631 | |
Sample type B | 1.823 | g | 32.802 | 0.261 | 8.555 | |
Sample type A | 1.905 | ref1 | 26.645 | 0.280 | 7.458 | 7.336 |
Sample type A | 1.886 | ref1 | 26.618 | 0.276 | 7.335 | |
Sample type A | 1.868 | ref1 | 26.579 | 0.271 | 7.215 | |
Sample type B | 1.918 | ref1 | 26.101 | 0.283 | 7.382 | 7.353 |
Sample type B | 1.906 | ref1 | 26.096 | 0.280 | 7.309 | |
Sample type B | 1.915 | ref1 | 26.105 | 0.282 | 7.368 | |
Sample type A | 1.900 | ref2 | 26.191 | 0.279 | 7.298 | 7.180 |
Sample type A | 1.881 | ref2 | 25.983 | 0.274 | 7.129 | |
Sample type A | 1.879 | ref2 | 25.962 | 0.274 | 7.113 | |
Sample type B | 1.890 | ref2 | 25.308 | 0.277 | 6.998 | 7.054 |
Sample type B | 1.883 | ref2 | 25.256 | 0.275 | 6.940 | |
Sample type B | 1.911 | ref2 | 25.689 | 0.281 | 7.223 | |
Sample type A Efficiency-weighted ΔC q | \( {\Delta C}_{q;r,A}^{(w)}= \)1.1387 | |||||
Sample type B Efficiency-weighted ΔC q | \( {\Delta C}_{q;r,B}^{(w)}= \)1.4077 |