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Table 2 Results of unpaired t-test. An unpaired t-test and 95% confidence interval are calculated in SPSS assuming unequal variances using the hypothetical data from Table 1 and three other hypothetical plate experiments. The P-value is from a two-tailed test assuming a mean difference of 0

From: A common base method for analysis of qPCR data and the application of simple blocking in qPCR experiments

 

Sample type A \( {\Delta C}_{q;r,A}^{(w)} \)

Sample type B \( {\Delta C}_{q;r,B}^{(w)} \)

 

r = 1

1.1387

1.4077

 

r = 2

0.845

1.291

 

r = 3

0.499

1.496

 

r = 4

0.699

1.172

 

Mean \( {\Delta C}_q^{(w)} \)

0.7954

1.3417

 

SD

0.269

0.141

 

N

4

4

 

\( {\Delta \Delta C}_q^{(w)} \)

  

−0.546

T for \( {\Delta \Delta C}_q^{(w)} \)

  

−3.60

P

  

0.019

95% CI for \( {\Delta \Delta C}_q^{(w)} \)

  

(−0.949, −0.143)

Estimated Expression Ratio \( {10}^{-\Delta \Delta {C}_q^{(w)}} \)

  

3.52

95% CI for \( {10}^{-\Delta \Delta {C}_q^{(w)}} \)

  

(1.33, 9.29)