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Table 4 Range of percent improvement using CONSTAX

From: CONSTAX: a tool for improved taxonomic resolution of environmental fungal ITS sequences

Taxnomic Ranka Percent Increaseb ITS1-Soil ITS2-Soil ITS1-Plant ITS2-Plant ITS1-BCc ITS2-BCc ITS1-UNc,d ITS2-UNc,d Mean Increase
Kingdom max. 0.00 0.00 (1.60) 0.00 (0.20) 0.00 0.00 (1.00) 0.00 (0.20) 0.00 (2.20) 0.00 0.81 (1.14)
min. 0.40 3.00 1.40 0.20 3.60 1.40 2.60 0.40
Phylum max. 0.47 1.29 4.46 (5.25) 2.84 6.05 1.70 5.57 (7.62) 2.76 6.83 (6.50)
min. 5.21 (4.03) 13.18 (5.43) 17.06 18.01 11.46 12.24 11.73 8.90
Class max. 1.58 1.83 0.93 4.89 3.04 0.84 3.98 3.70 8.56 (5.36)
min. 9.23 (2.11) 24.77 (7.65) 21.98 (18.27) 26.63 (22.28) 18.26 (8.70) 9.28 (5.49) 13.94 (5.98) 9.26 (5.19)
Order max. 1.69 2.47 0.33 2.58 2.48 1.40 5.75 3.23 10.94 (5.73)
min. 11.83 (4.79) 27.21 (6.71) 37.42 (20.86) 42.58 (24.52) 19.31 (7.92) 7.44 (5.58) 19.91 (8.85) 11.29 (4.03)
Family max. 1.36 3.54 2.47 3.16 1.86 1.10 6.88 6.13 15.72 (6.43)
min. 13.9 (1.69) 30.81 (6.57) 58.02 (22.63) 61.05 (26.32) 20.50 (9.94) 11.60 (6.08) 32.80 (8.99) 27.83 (7.08)
Genus max. 2.56 6.25 2.51 5.33 2.40 1.89 9.15 9.03 27.06 (9.04)
min. 28.21 (3.85) 53.13 (8.59) 88.94 (37.69) 85.33 (36.00) 31.20 (7.20) 35.22 (10.69) 63.38 (10.56) 62.58 (9.03)
Species max. 5.65 8.51 2.70 1.92 3.19 1.83 9.28 13.68 34.65 (13.20)
min. 52.42 (16.13) 65.96 (14.89) 98.65 (47.97) 96.15 (51.92) 41.49 (9.57) 51.38 (21.10) 81.44 (11.34) 89.47 (17.89)
  1. aPercent improvement calculated with RDP, SINTAX, and UTAX outputs (numbers in paranthesis calculated without including UTAX, only differing values displayed). Ranges represent minimum and maximum improvement when compared to all three classifiers at a given level
  2. bEquation to calculate percent increase, where N = assigned OTUs. \( \frac{\max\ or\ \min\ N}{consensus\ N}\times 100 \)
  3. cReads are forward (ITS1) or reverse (ITS2), not merged read pairs
  4. dDataset was processed with denoising instead of clustering