Fig. 4

Anti-DENV activities of boceprevir, telaprevir, and asunaprevir by plaque formation assays. Cultured BHK cells were infected with DV2-NGC virus (from Huh7 cell supernatant), treated with DMSO and different concentrations of the inhibitor candidates. After incubation, the viral plaques were quantified and the count (PFU/ml) was recorded. a Viral replication is observed by the fold change decrease in plaque count (PFU/ml) on addition of different inhibitor concentrations compared to DMSO control. The statistically significance by one-tailed paired T-test (n = 3) is shown [*P < 0.05; **P < 0.01]. b Dose vs %inhibition curves were plotted and the EC₅₀ values of asunaprevir and telaprevir were observed to be 10.4 μM and 24.5 μM, respectively for DV2-NGC