Fig. 1

A flow-chart of the steps used by Spherical. The blue circle encompasses all processes that are carried out in an iterative manner until the results meet the ‘user defined criteria’. The ‘user defined criteria’ is defined as any user option which indicates a point at which Spherical should stop iterating. The arrows width indicates the possible decrease in file size depending on user sub-sample selection. 1; User input data (usually quality controlled sequencing files). 2; Spherical takes a random subset of the input sequencing data. The size of the subset is determined by user. 3; An assembly of the subset is generated. 4; The number of reads aligning to the combined assembly are determined. 5; If the number of reads aligning meets the user criteria Spherical will move to step 7, otherwise Spherical will continue to step 6. 6; Reads that do not align to the combined assembly are used as input for the next round. 7; Spherical exits and combines the individual iterations assemblies into a single file