Oasis 2: improved online analysis of small RNA-seq data

BMC Bioinformatics

Table 4 Overlap of differentially expressed sRNAs using three datasets

	Statistic¹	Overlap²	Validated overlap³	FP overlap⁴
AD	Wilcoxon-Mann-Whitney	60%	75%(6/8)⁵	0% (0/2)
Psoriasis	Pearson’s chi-squared	73%	64% (7/11)	0% (0/1)
Renal Cancer	edgeR [14]	76%	80% (4/5)	NA
Schizophrenia	DESeq2 (Dejian et al., 2015)	41%	67%(2/3)	0% (0/1)

¹Oasis 2 uses a negative binomial distribution as basis for its statistical evaluation of the differential expression. A very similar approach is taken by the edgeR package that has been used in the Renal Cancer study. The Psoriasis data was analyzed using a Pearson’s chi-squared test and the AD dataset was analyzed using the non-parametric Wilcoxon-Mann-Whitney test. Schizophrenia dataset used the same approach like Oasis 2. ²Overlap of differentially expressed miRNAs comparing Oasis 2’s results to published data. The percentage is calculated in reference to the shorter DE list. ³Overlap of differentially expressed miRNAs that have been validated independently in addition to the sRNA-seq experiment. ⁴False positive (FP) differentially expressed miRNAs detected by Oasis 2. ⁵Only known validated DE miRNAs are considered

ISSN: 1471-2105