Fig. 2

Estimated context-specific substitution error rates for polymerase Hi-Fi 2X. a) R1 reads. b) R2 read. c) Γ, the ratio of error rate in R1 over R2. P-values were computed by performing a two-tailed z-test. d) Δ, the difference between their corresponding Phred quality scores. We reduced the depth of paired-end reads to approximately 1,300,000 × through an in silico depth reduction procedure. Results were obtained by averaging over 100 independent samples to establish error bars which indicate one standard deviation from the average