Fig. 4
From: MERIT reveals the impact of genomic context on sequencing error rate in ultra-deep applications
Significant variation in error rates for possible amino acid changes at individual codons. a) Six frequently mutated residues in the TP53 gene. b) Two hotspot residues in the SF3B1 gene. The higher the rate of error for a specific base change, the lower the power to distinguish true mutations from sequencing artifacts at its position. Here, the error rates represent the amplification by the Hi-Fi 2X polymerase. Error bars represent one standard deviation from the mean of 100 independent sub-samples