Clustering of circular consensus sequences: accurate error correction and assembly of single molecule real-time reads from multiplexed amplicon libraries

Table 1 Comparison of LAA and C3S-LAA consensus sequences for B73 amplicons

Library type^a	Method	Number of consensus sequences	Complete match^b (100% identity)	Truncated match (100% identity)	Partial match (<100% identity)
Single	LAA	14	7	1	6
Single	C3S-LAA	9	9	0	0
Multiplex	LAA	8	4	1	3
Multiplex	C3S-LAA	6	5	0	1

^aThe single library had nine expected consensus sequences, whereas the multiplex library had six expected consensus sequences.
^bFor the multiplex sample library, the B73 v3 assembly contained a gap relative to one of the five amplicon sequences, leading to one C3S-LAA sequence having a partial match. This gap was filled in the latest B73 v4 release

ISSN: 1471-2105