Fig. 3From: A novel computational method for automatic segmentation, quantification and comparative analysis of immunohistochemically labeled tissue sectionsSteps of the tissue region segmentation procedure. a original image depicting human tonsil marked Ki-67 antibody; b gray level image after the heavy filtering process; c segmented tissue region before its refinement by applying morphological operators; d segmented tissue region after morphological “cleaning” and holes filling; e RGB image included in the tissue region; f segmented markersBack to article page