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Fig. 3 | BMC Bioinformatics

Fig. 3

From: A novel computational method for automatic segmentation, quantification and comparative analysis of immunohistochemically labeled tissue sections

Fig. 3

Steps of the tissue region segmentation procedure. a original image depicting human tonsil marked Ki-67 antibody; b gray level image after the heavy filtering process; c segmented tissue region before its refinement by applying morphological operators; d segmented tissue region after morphological “cleaning” and holes filling; e RGB image included in the tissue region; f segmented markers

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