Fig. 1

Overview of methods steps. Schematic overview of rscreenorm steps. Illustrations use the arrayed whole-genome lethality siRNA screen on cell line 786-O (3 replicates). a Raw (log2-transformed) viability empirical distributions, separately for library features, negative and positive controls, with between-replicates differences in functional range and data distributions illustrated by pink-dashed and green-solid lines, respectively. b Densities of lethality scores, representing the phenotype relative to the assay controls. These make data values and functional ranges more comparable, but differences between data distributions (in purple lines) remain. c a core set of lethality scores (dark-gray dashed lines) is chosen per replicate. d Distributions of rscreenorm scores, where most differences between replicates have been corrected for