Fig. 4
From: PIPEBAR and OverlapPER: tools for a fast and accurate DNA barcoding analysis and paired-end assembly
Example of an erroneous sequence generated by SeqTrace. Sequences PCCMN351-FWD and PCCMN351-REV are the trimmed sequences that should overlap, originated from the Dataset 1. SeqTrace erroneously insert gaps (from base 100 to 500) that connect the two sequences without having any insert distance information. The figure was generated using Geneious by aligning the PCCMN351-FWD and PCCMN351-REV sequences to its respective barcode generated by SeqTrace. The qualities of the forward and reverse sequences are demonstrated as a histogram. As SeqTrace does not generate a FASTQ file, we could not evaluate the quality of the barcode generated