Skip to main content

Table 3 Numerical values of the efficiency, coverage and matching-bias scores for the three selected primers assessed on GreenGenes and SILVA reference sequences

From: Optimizing PCR primers targeting the bacterial 16S ribosomal RNA gene

Representative set

Score

Primer-set-pair 1

Primer-set-pair 2

Primer-set-pair 3

GreenGenes 85%

Efficiency

9.66

9.93

10

Coverage

0.863

0.854

0.833

Matching-bias

0.45

0.44

0.51

GreenGenes 99%

Coverage

0.969

0.963

0.954

Matching-bias

0.22

0.22

0.27

SILVA 99%

Coverage

0.974

0.969

0.962

Matching-bias

0.20

0.19

0.24

  1. The scores have been computed with respect to the GreenGenes 85% representative set, the GreenGenes 99% set and the SILVA 99% not redundant set. Efficiency ranges from 0 to 10 and coverage ranges from 0 to 1
Back to article page

BMC Bioinformatics

ISSN: 1471-2105

Contact us