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Table 4 Top-five assemblies by biological structure for P. xanthii with their respective mean distances from references

From: TransFlow: a modular framework for assembling and assessing accurate de novo transcriptomes in non-model organisms

Assembly ID

Module 1

Module 2

Module 3

MD

Haustorium

 scOases_cat_cd_rcMin2

 

Concatenation of the two OASES-scaffolded assemblies with different kmers, sequence redundancy removal and Minimus2 reconciliation

  

0.3848

 scOases_cat

 

Concatenation of the two OASES-scaffolded assemblies with different kmers

  

0.3850

 scOases_cat_cd

 

Concatenation of the two OASES-scaffolded assemblies with different kmers and sequence redundancy removal

  

0.3861

 scSoap_cat_cd

 

Concatenation of the two SOAP-scaffolded assemblies with different kmers and sequence redundancy removal

  

0.3887

 scOasesK35

 

OASES-scaffolded assembly with k-mer 35

  

0.3915

Epiphytic structures

 ctMIRA_ctEulK29_rcCAP3

  

CAP3-reconciled assembly

 

0.0558

 ctMIRA

  

MIRA4-primary assembly

 

0.0688

 ctEulK29

  

EULER-SR primary assembly with k-mer 29

 

0.1435

Comprehensive transcriptome

 arMIRA/scSoap_cat_cd_rcMin2

 

Concatenation of the two SOAP-scaffolded assemblies with different kmers, sequence redundancy removal and Minimus2 reconciliation

Pre-processed reads

Assembly-reads combination using MIRA4

0.0470

 arMIRA/ctSoapK25

 

SOAP-primary assembly with k-mer 25

Pre-processed reads

Assembly-reads combination using MIRA4

0.0473

 arMIRA/scRay_cat_cd_rcMin2

 

Concatenation of the two RAY-scaffolded assemblies with different kmers, sequence redundancy removal and Minimus2 reconciliation

Pre-processed reads

Assembly-reads combination using MIRA4

0.0476

 arMIRA/ctRayK35

 

RAY-primary assembly with k-mer 35

Pre-processed reads

Assembly-reads combination using MIRA4

0.0479

 arMIRA/ctSoap_cat_cd_rcMin2

 

Concatenation of the two SOAP-primary assembling kmers, sequence redundancy removal and Minimus2 reconciliation

Pre-processed reads

Assembly-reads combination using MIRA4

0.0479