Fig. 2

a Epithelial and mesenchymal scores obtained from singscore for the TCGA breast cancer samples (hexbin density plot) and a collection of breast cancer cell lines (circle markers, coloured by subtype). Note that as per the original study by Tan et al., the epithelial and mesenchymal signatures are distinct (but overlapping) for tumours and cell lines; b Differences in epithelial and mesenchymal scores for 32 overlapping breast cancer cell lines between Daemen et al. and the CCLE datasets. The majority of cell lines show relatively consistent scores in these two data sets (circled in the lower left corner); c The HCC1428 cell line has very similar scores in each dataset, while the MDA-MB-231 cell line has a large shift in epithelial score, and the HCC202 cell line has a large shift in mesenchymal score; d Three microarray samples from the TGFβ- EMT data set [8] with low, medium and high scores for the TGFβ-EMT signature; e Scatter plots demonstrating the relationship between rank dispersions (MAD) and scores obtained by singscore, for: total score (combined up- and down-set scores), distinct expected up-regulated gene set scores, and distinct expected down-regulated gene set scores