Fig. 2
From: Automated cell segmentation in FIJI® using the DRAQ5 nuclear dye
Exemplary images and shape descriptors for HeLa and differentiated THP-1 cells. a Brightfield (transmitted laser light) image of HeLa with segmentation lines (red) produced by our algorithm. b Brightfield (transmitted laser light) image of differentiated THP-1 with segmentation lines (red) produced by our algorithm. c Mean projected cell area of differentiated THP-1 and HeLa cells shows that THP-1 cells have a 36% smaller area compared to HeLa. d Quantification of cell circularity confirms that THP-1 cells have a 41% higher (mean) circularity value compared to HeLa. e Elongated HeLa cells show a mean aspect ratio above 2 while the round shape of THP-1 is reflected by an aspect ratio ~ 1. Data are shown as mean ± standard error of mean (sem). **** indicates p < 0.0001 (t-test). For HeLa, 129 cells in n = 8 images from two pooled experiments (the same images as in Fig. 1) were analyzed; for THP-1, 135 cells in n = 13 images from two pooled experiments were analyzed. Scale bar is 200 μm in both images