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Table 2 Top ideal GRIBCG-generated sgRNAs for each chromosome in A. thaliana and C. elegans

From: GRIBCG: a software for selection of sgRNAs in the design of balancer chromosomes

Organism Chromosome sgRNA Sequences SSV Average On-Target CFD Number of Cuts Coverage (%)
A. thaliana 1 TATGGCTTAAAATTTTATGTGGG 0.341 0.821 2.4 7 82
A. thaliana 2 TTTAACCCACGGTATACCGCGGG 0.577 0.754 1.3 4 75
A. thaliana 3 CAGATAGAGAGAAAGAGAGAGGG 0.252 0.909 3.3 6 83
A. thaliana 4 AGGGCAAAAAAAAAAAACAGAGG 0.325 0.965 2.7 5 88
A. thaliana 5 GGAGTAGGAGGAAGAGGAGGAGG 0.385 0.962 2.6 7 100
C. elegans I GAGGGGGGGCCATACTAATAGGG 0.958 0.642 1.0 4 96
C. elegans II TAGTGAAATTTTCATGAAAATGG 0.786 0.587 1.0 4 79
C. elegans III CCTGGAGCTCCTGGAGCTCCTGG 0.949 0.675 1.0 4 95
C. elegans V TGGAATAAAAATCTCAGAAATGG 0.757 0.807 1.0 4 76
C. elegans X TACATTTTAATTTTAAAAAAAGG 0.903 0.522 1.0 4 90