Fig. 2

a SpliceV plot of RNA coverage and splicing from normal stomach tissue (wild type TP53). b SpliceV plot of a gastric tumor with a 1 base (T) deletion at a splice acceptor (chr17:7673610, HG38 genome build), disrupting the splicing from exon 8–9 and causing the utilization of a novel cryptic downstream splice acceptor at position chr17:7673590; resulting in a frameshift deletion. c SpliceV plot of a gastric tumor with a G > A splice donor variant at chr17:7675993. Part of the intron is retained (chr17:7675884–7,675,993) and a novel intronic splice donor site is utilized, with the same upstream acceptor. This introduces a frameshifting insertion into the protein coding sequence. Asterisks indicate the SNV location and insets are enlarged representations of the transcript structure. Nucleotide sequences at the cryptic splice sites are labeled, with the junctions occurring between the red and black bases in each figure. These samples were initially provided by The Cancer Genome Atlas [2], with alignments obtained from the Genomic Data Commons [9]