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Table 1 Estimation of distribution normality

From: Pairwise efficiency: a new mathematical approach to qPCR data analysis increases the precision of the calibration curve assay

Dilution set (wells) Skew Kurtosis Total data points
A1–6 1.064 7.357 237
B1–6 0.615 4.085 237
C1–6 0.221 3.556 244
D1–6 1.051 6.305 241
E1–6 0.473 5.524 240
F1–6 1.88 6.769 222
G1–6 2.012 10.079 220
H1–6 1.379 12.177 223
A7–12 −0.337 2.16 220
B7–12 0.098 4.508 217
C7–12 0.215 2.838 259
D7–12 0.739 2.514 241
E7–12 0.563 3.555 223
F7–12 −0.034 3.843 206
G7–12 1.429 7.023 198
H7–12 −0.148 5.319 240
  1. Pairwise E values of 16 dilution sets were analyzed for skewness and kurtosis. Skewness values that deviate from 0 indicate asymmetry of the distribution, making it a non-normal distribution. Positive kurtosis values also imply deviation from normal distribution and indicate that the distribution is sharp (more values are close to mathematical expectation, and precision is higher than would be expected in the case of normal distribution). The right column contains the numbers of individual pairwise E determinations for each dilution set that were taken for this analysis