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Table 1 Estimation of distribution normality

From: Pairwise efficiency: a new mathematical approach to qPCR data analysis increases the precision of the calibration curve assay

Dilution set (wells)

Skew

Kurtosis

Total data points

A1–6

1.064

7.357

237

B1–6

0.615

4.085

237

C1–6

0.221

3.556

244

D1–6

1.051

6.305

241

E1–6

0.473

5.524

240

F1–6

1.88

6.769

222

G1–6

2.012

10.079

220

H1–6

1.379

12.177

223

A7–12

−0.337

2.16

220

B7–12

0.098

4.508

217

C7–12

0.215

2.838

259

D7–12

0.739

2.514

241

E7–12

0.563

3.555

223

F7–12

−0.034

3.843

206

G7–12

1.429

7.023

198

H7–12

−0.148

5.319

240

  1. Pairwise E values of 16 dilution sets were analyzed for skewness and kurtosis. Skewness values that deviate from 0 indicate asymmetry of the distribution, making it a non-normal distribution. Positive kurtosis values also imply deviation from normal distribution and indicate that the distribution is sharp (more values are close to mathematical expectation, and precision is higher than would be expected in the case of normal distribution). The right column contains the numbers of individual pairwise E determinations for each dilution set that were taken for this analysis