Pairwise efficiency: a new mathematical approach to qPCR data analysis increases the precision of the calibration curve assay

Table 2 Comparison of the calibration curve method with the Pairwise Efficiency method

Approach	SD	Max E	Min E	Max-Min difference	Average E
Calibration curve	0.019	0.83	0.76	0.072	0.80
Pairwise Efficiency	0.010	0.82	0.78	0.047	0.80

Standard deviations (SD) obtained from the Monte Carlo test, maximal and minimal efficiency values, the range between maximal and minimal values, and the average efficiencies are shown. While the average E value was the same for both methods (E = 0.80), the precision of E estimation obtained by the Pairwise Efficiency method, expressed as standard deviation (SD), was nearly two times higher, and the dispersion, expressed as the difference between maximal and minimal calculated E values, was 1.6 times smaller

ISSN: 1471-2105