Fig. 5

Detection cultivar specific deletion. a The bidirectional alignment of 1287 bp deletion in ‘Koshihikari’. The deletion does not exist in ‘Nipponbare’, ‘Aichinokaori’, ‘Akitakomachi’, ‘Hinohikari’, ‘Hitomebore’ and ‘Kinuhikari’. b A diagram of primers and estimated product size. c Detection of deleted region using primer from the deleted region. Lane M; 100 bp ladder, 1; ‘Nipponbare’, 2; ‘Koshihikari’, 3; ‘Akitakomachi’, 4; ‘IR64’, 5; ‘Aichinokaori’, 6; ‘Asahinoyume’, 7; ‘Oborozuki’, 8; ‘Kinuhikari’, 9; ‘Kirara397’, 10; ‘Koshiibuki’, 11; ‘Sainokagayaki’, 12; ‘Sasanishiki’, 13; ‘Tsugaruroman’, 14; ‘Nanatsuboshi’, 15; ‘Haenuki’, 16; ‘Hatsushimo’, 17; ‘Hanaechizen’, 18; ‘Hitomebore’, 19; ‘Hinohikari’, 20; ‘Fusakogane’, 21; ‘Massigura’, 22; ‘Yamadanishiki’, 23; ‘Yumetsukushi’, 24; ‘Yumepirika’. d Detection of contamination. Sequential diluted ‘Nipponbare’ DNA was mixed to 10 ng ‘Koshihikari’ template DNA. Lane M; 100 bp ladder, 1; 10 ng, 2; 5 ng, 3; 2.5 ng, 4; 1.25 ng, 5; 0.63 ng, 6; 0.31 ng, 7; 0.16 ng, 8; 0,08 ng, 9; 0.04 ng, 10; 0.02 ng ‘Nipponbare’ DNA in 10 ng ‘Koshihikari’ DNA