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Fig. 3 | BMC Bioinformatics

Fig. 3

From: Magic-BLAST, an accurate RNA-seq aligner for long and short reads

Fig. 3

Actual read length histograms for three experimental sets as well as iRefSeq. All Illumina reads have the same length (101 + 101 bases, paired-end) but other sets have non-trivial length distributions. Newer sequencing technologies tend to produce longer sequences. PacBio has the longest reads of the experimental sets, ranging from 710 to 2703 bases (average 1854 bases). Roche 454 Titanium reads span from 33 to 808 bases, with an average of 348 bases. iRefSeq has the longest reads of all sets: these full-length mRNAs range in length from 147 bases to 109,224 bases, with an average of 3427 bases. 9900 are longer than 10 kb. The Y-axis presents the number of reads per 100-base bin (on the X-axis). The Y scale is reduced 100 times for Roche 454, and 50,000 times for Illumina, which has the highest throughput of all technologies

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