Fig. 2

Evaluation of the circular DNA detection methods. a-d Circle-Map (orange), CIRCexplorer2 (blue), Circle_finder (green) and Circle-Map with no realignment (grey) were evaluated on simulated circular DNA datasets with varying sequencing depths (e-g) and on real circle enriched data from human muscle. a Sensitivity at 30X and b 7.5X measured as the number of called circles found in the simulation set divided by the total number of simulated circles. Precision at (c) 30X (d) and 7.5X measured as the number of correctly called circles divided by the total number of called circles, true and false. e Histogram with the percentage of bases covered by sequencing reads for every circular DNA detected. The number of breakpoint reads (e.g. split and discordant reads) relative to the mean sequencing coverage within the circular DNA coordinates for f Circle-Map, g CIRCexplorer2 and h Circle_finder