Fig. 1

ARTDeco evaluates different aspects of readthrough transcription. a Schematic diagram of typical transcription termination (top) and readthrough transcription (bottom). b Total RNA-seq, RNA polymerase II ChIP-seq, and H3K27ac ChIP-seq data at IFIH1 locus. Normalized read coverage ranges are indicated on the right and signals exceeding these levels may be clipped (e.g. RNA-seq coverage on the exons of IFIH1). IFIH1 represents a primary induction gene while FAP, GCG, and DPP4 represent read-in genes. c Schematic depicting the regions used to quantify read-in levels, readthrough levels, and DoG transcript discovery for each gene