Fig. 2

Quantification of readthrough phenotypes in IAV-infected monocyte derived macrophages. a Distribution of read-in levels (log2 ratio of reads in read-in region vs. gene body) for top 1000 expressed genes. b Distribution of readthrough levels (log2 ratio of reads in downstream region vs. gene body) for top 1000 expressed genes. c Downstream gene read-in level vs. upstream gene read-in level in the first replicate of the IAV condition. Both downstream and upstream genes were expressed at a level > 0.25 FPKM (r = 0.55; p < 1e-151). d Distribution of DoG lengths for DoGs discovered by ARTDeco using default settings (minimum length of 4 kb, window size of 500 bp, and minimum read density of 0.15 FPKM)