Fig. 5

ARTDeco analysis of readthrough transcription induced by heat shock in NIH 3T3 cells. a Total RNA-seq levels at the Hsp90aa1 locus in mouse fibroblasts for heat shock and mock conditions from Vilborg et al. [32]. Hsp90aa1 represents the primary induction genes while 1700001K19Rik is a read-in gene defined by ARTDeco. b Distribution of read-in levels for top 1000 expressed genes following heat shock. c Distribution of readthrough levels for top 1000 expressed genes following heat shock. d Distribution of DoG lengths in both mock and heat shock conditions. e Heat map of GO term enrichment (−log10 p-value) for top 15 enriched GO terms for primary induction and read-in genes. f HOMER motif enrichment for primary induction and read-in genes