Fig. 2

Agarose gels showing amplified PCR products for the TraesCS5A01G531300 gene using primers designed by AutoCloner. Details of the primers are shown in Table 1. The DNA ladder used was the Quick-Load® Purple 2-log DNA ladder, manufactured by New England Biolabs, containing DNA fragments ranging from 0.1 kb to 10 kb in size. The expected product sizes for T.300.577–1989, T.300.1904–3138, T.300.2632–3437 and T.300.2888–4219 were 1412, 1234, 805 and 1331 bases respectively. A subsequent PCR (not shown) in which the annealing temperature was increased from 58 °C to 60 °C increased the specificity of the T.300.2888–4219 set of primers