Table 1 List of primers designed by AutoCloner to amplify and sequence TraesCS5A01G531300 in Apogee. Oligo names succeeded by an F are forward primers, whilst an R indicates a reverse primer. Both primers for PCR and primers for Sanger sequencing are included
From: AutoCloner: automatic homologue-specific primer design for full-gene cloning in polyploids
Oligo Name | Sequence (5′- > 3′) | Type |
|---|---|---|
T.300.577–1989.F | AGACTTCCTGAACACGGCACAA | PCR |
T.300.577–1989.R | CTTCTTGATGGCGCGGCATATAT | PCR |
T.300.1904–3138.F | TAGGTTGACGTCATCGAAGCAG | PCR |
T.300.1904–3138.R | CGGGTGAGAAGCAAGGACTC | PCR |
T.300.2632–3437.F | CCATGGTGAGGTTGAGGTCC | PCR |
T.300.2632–3437.R | GGTGCAGCAAGAGTACGGAG | PCR |
T.300.2888–4219.F | CGGACGATGACAACAGGGAG | PCR |
T.300.2888–4219.R | CCCTGCTCTCTCTCTCTCTCT | PCR |
T.300.577–1989.split2.F | CTCGAACTCGCTATTGGGCT | Sanger |
T.300.577–1989.split3.F | AGTCAAGGTACAATATGTGACTGA | Sanger |
T.300.1904–3138.split2.F | CCGTGAAGTACCGAAACCCA | Sanger |
T.300.1904–3138.split3.F | TAACGAACCTGGTGCCTTCG | Sanger |
T.300.2632–3437.split2.F | TCAGGTCCTTGGCCAGTTTC | Sanger |
T.300.2888–4219.split2.F | GTGGAGACATGGAGGAGCAC | Sanger |
T.300.2888–4219.split3.F | ACCAACACTCAAGCAAAGGGA | Sanger |