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Fig. 8 | BMC Bioinformatics

Fig. 8

From: METROID: an automated method for robust quantification of subcellular fluorescence events at low SNR

Fig. 8

ROI standardization sequence. a Bright field rat cardiomyocyte image overlaid by contour representing cell mask. b Outer region division: aligned cell outer contour (black contour), mid contour (gray contour) and inner contour (light gray contour). Dashed red lines indicate ROI separation, black dots indicate a segment beginning and blue crosses indicate closest outer and inner contour points. Green dot is the first segment position. c Inner region division: aligned outer contour (black contour) and inner contour (light gray contour). Dashed red and blue lines indicate ROI separation. d Standardized ROIs represented by different numbers and colors with original cell mask represented by yellow contour

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