Fig. 8
From: Multi-omics profiling reveals microRNA-mediated insulin signaling networks
Validation of the two target genes Crem and Fn1 of miR-146b that are common in prediction and RNA-seq analysis. a Predicted target sites of miR-146b in 3′-UTR of rat Crem and Fn1. b mRNA expression analysis of two target genes of miR-146b by qRT-PCR c Ago2-IP analysis was carried out to measure relative enrichment of Crem and Fn1 genes of miR-146b as measured by qRT-PCR. src-scramble. d Luciferase activity in INS1 cells cotransfected with either empty luciferase vector pmiR-GLO as positive control or the construct containing 3’UTR of two target genes Crem and Fn1 and miR-146b mimics (mimic) or scramble control (scr)