Fig. 1
From: MAIRA- real-time taxonomic and functional analysis of long reads on a laptop
MAIRA workflow. In an online manner, a batches of long reads obtained from a sequencing device are subjected to protein alignment against a precomputed database of genus-specific marker genes. Based on this, b the list of detected genera is updated in real-time. c Substantial evidence for the presence of a specific genus triggers d batch-wise protein alignment against a full database of proteins associated with the genus. Based on this, a protein graph is updated and used to perform species and-strain level analysis of the long reads, including the identification of antibiotic resistance and virulence factors