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Fig. 3 | BMC Bioinformatics

Fig. 3

From: Brain antibody sequence evaluation (BASE): an easy-to-use software for complete data analysis in single cell immunoglobulin cloning

Fig. 3

Workflow and implementation of cBASE: After quality control, PCR-amplicon and plasmid sequencing files are parsed, and then blasted against germline VDJ-genes using IgBLAST. Ig gene variable regions are compared nucleotide by nucleotide and antibody expression is recommended if no amino acid changing mutations are detected

CDR = complementarity determining region. Ig = immunoglobulin.

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