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Table 1 Validation of aBASE. Number of correctly identified parameters using automated aBASE evaluation compared to the results of manual analysis

From: Brain antibody sequence evaluation (BASE): an easy-to-use software for complete data analysis in single cell immunoglobulin cloning

Parameter analyzed

Total number of chains analyzed

Correctly analyzed chains

Comments

VDJ

181

100,00%

 

Ig Isotype

96

97,92%

2 chains were correctly analyzed by aBASE as IgM Isotype, but results flagged as low-confidence

Ig Functionality

181

100,00%

 

Cloning recommendation

181

100,00%