Table 2 Description of the navigation buttons from where users can begin playing the audio and visual displays

Button set 1

RNA features associated with coding regions

5′UTR

5′ untranslated region

Poly-/-protein

Two buttons representing the coding region before and after the -1 frameshift position of the large polyprotein

9 U regions

Each navigates to an untranslated region between ORF’s

-S-

Region coding for the canonical S protein

-E-

Region coding for the canonical E protein

-M-

Region coding for the canonical M protein

-N-

Region coding for the canonical N protein

ORF 3a, ORF 6, ORF 7a, ORF 7b, ORF 8, ORF 10

Regions thought to code for other proteins or polypeptides

3′UTR

5′ untranslated region

Button set 2

RNA features associated with the NSP proteins

5′UTR

5′ untranslated region

N1—N16

Location of the 16 NSP proteins within the large polyprotein

14 C sites

Cleavage sites within the translated polyprotein giving rise to the 16 individual NSP proteins

S—ORF 10

Region of the RNA sequence downstream of the polyprotein

3′UTR

5′ untranslated region

Button set 3

RNA features associated with the TRS regions

5′UTR

5′ untranslated region

T1—T10

Location of TRS 1 to TRS 10. TRS1 is sometimes referred to as the leader TRS and is linked to the subsequence TRS 2—10 to produce the sub-genomic regions during transcription

5 SL regions

Stem Loop regions giving rise to structured regions of RNA. These are formed due to sequence complementarity and base pairing

12 Seq regions

Undefined sequences between the TRS regions, these often correspond closely to the ORF regions

3′UTR

5′ untranslated region