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Table 4 The results of test micrographs scaling operations

From: DeepCryoPicker: fully automated deep neural network for single protein particle picking in cryo-EM

Dataset Original micrograph dimension Original particle size Scaling operation and best factor Scaled particle size Scaled micrograph dimensions Unified micrograph size
Apoferritin top-view 1200 × 1240 94 × 94 Up-sampling (2.375) 224 × 224 3373 × 3373 3374 × 3374
KLH (top-view) 2048 × 2048 187 × 187 No-scaling 187 × 187 2048 × 2048 2048 × 2048
KLH (side-view) 2048 × 2048 221 × 221 No-scaling 221 × 221 2048 × 2048 2048 × 2048
Ribosome
Ribosome irregular shape
4096 × 4096 320 × 320 Down-sampling (0.625) 200 × 200 4030 × 4030 4030 × 4030
β-Galactosidase complex particle shape 4096 × 4096 188 × 188 Up-sampling (1.125) 212 × 212 4608 × 4608 4608 × 4608
  1. The second column shows the original test micrographs dimensions, the third column the detected particle patches’ coordinates (dimensions) using AutoCryoPicker [24] and SuperCryoEMPicker [25], the fourth column the selected scaling operation and best factor, the fifth column the scaled particle size using the selected scaling factor, the sixth column the scaled micrograph dimensions, and the last column the unified micrograph size