Fig. 2

Simulation result. a Evaluation of the method using simulated datasets based on the S. pombe transcriptome; only transcripts that contain at least 3 introns are considered (n = 709). (Left violin) Evaluation of the method using simulated short-read pair-end sequencing (read length = 150). (Middle violin) Evaluation of the method using simulated long-read sequencing (read length = ~ 800). (Right violin) Evaluation of the method using simulated super long-read sequencing (read length = ~ 5000). b Simulation result for humans; a total of 226 multi-introns (intron number \(\ge 3\)) containing transcripts are used. (Left violin) Mate-pair sequencing, the fragments size = ~ 15,000 and the read length = 150. (Middle violin) Long-read sequencing, read length = ~ 800 bp. (Right violin) Super long-read sequencing, read length = ~ 12,000. c Simulating the intron splicing order read count matrix 1000 times by giving random orders and then randomly erasing some values in the intron splicing order read count matrix. The percentage of intron splicing order pairs retained is labelled above each violin plot