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Fig. 1 | BMC Bioinformatics

Fig. 1

From: High quality genome assemblies of Mycoplasma bovis using a taxon-specific Bonito basecaller for MinION and Flongle long-read nanopore sequencing

Fig. 1

Implementation of custom-pg45 Bonito training in reference-based (a) and de novo genome assembly bioinformatic workflows (b). The newly generated custom-pg45 Bonito training model was implemented in both reference-based (a) and de novo assembly (b) bioinformatics workflow. While for MiSeq short reads (purple), UniCycler (SPAdes-based) de novo assembler was used, long reads were used in a bioinformatics pipeline with either the Canu (orange) or Flye (blue) de novo assembler, supplemented with or without four rounds of Racon polishing. c MiSeq sequencing results in a highly gapped de novo assembled M. bovis PG45 genome as compared to MinION long read assemblies. A completeness of 100% indicates all genomic markers (n = 226 for Mycoplasma spp.) were present. A 100% Genome Fraction indicates the full M. bovis PG45 type strain genome was covered

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BMC Bioinformatics

ISSN: 1471-2105

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