Fig. 1
From: Auto3DCryoMap: an automated particle alignment approach for 3D cryo-EM density map reconstruction
The general workflow of the Auto3DCryoMap. a The general workflow of the Auto3DCryoMap. The green part of the workflow shows the micrographs data preprocessing. The red part of the workflow shows the fully automated single-particle picking using DeepCryoPicker [10]. The blue part of the workflow shows the general flow of the good 2D single-particle selection using unsupervised learning and deep learning classification. The yellow part of the workflow shows the fully automated particle alignment. The gray part shows the 3D density map reconstruction using single-particle reconstruction. b Simulated top-view (circular) of the Apoferritin molecule shapes. c Apoferritin real-world top-view (circular) protein shape. d Simulated top-view (circular) of the KLH molecule shapes. e KLH real-world top-view (circular) protein shape. f Simulated side-view (square) KLH molecule shape. g KLH real-world side-view (circular) protein